DNA fragments formed by the use of restriction endonucleases are separated by gel electrophoresis. (i) DNA fragments are negatively charged molecules. Thus, they move towards the anode under electric field through the medium. (ii) DNA fragments separate according to their size due to seiving effect of agarose gel. (iii) The separated DNA fragments can be viewed by staining the DNA with ethidium bromide followed by exposure to UV radiation. (iv) The separated bands of DNA are cut and extracted from gel piece. This is known as elution
How are the DNA fragments separated and isolated for DNA fingerprinting? Explain.
Answers
Ashwini kumar
DNA fragments formed by the use of restriction endonucleases are separated by gel electrophoresis.
(i) DNA fragments are negatively charged molecules. Thus, they move towards the anode under electric field through the medium.
(ii) DNA fragments separate according to their size due to seiving effect of agarose gel.
(iii) The separated DNA fragments can be viewed by staining the DNA with ethidium bromide followed by exposure to UV radiation.
(iv) The separated bands of DNA are cut and extracted from gel piece. This is known as elution