The separated DNA fragments are stained with ethidium bromide. (i) By the exposure to UV radiation, the separated DNA fragments become visible as orange-coloured bands. (ii) The separated bands of DNA are cut out from the agarose gel and DNA is extracted from these gel pieces, this process is called elution.
How are the DNA fragments separated by gel electrophoresis visualised and separated for use in constructing recombinant DNA?
Answers
Abhishek Nair
The separated DNA fragments are stained with ethidium bromide.
(i) By the exposure to UV radiation, the separated DNA fragments become visible as orange-coloured bands.
(ii) The separated bands of DNA are cut out from the agarose gel and DNA is extracted from these gel pieces, this process is called elution.