The insertional activation of J3-galactosidase enzyme, i.e. by inserting the desired gene in the coding region of enzyme, results in inactivation of (3-galactosidase gene in recombinants. The recombinant on transformed hosts are unable to produce any colour when grown on chromogenic substrate, thus acting as a selectable marker to differentiate recombinants from non-recombinants.
How is insertional inactivation of an enzyme used as a selectable marker to differentiate recombinants from non-recombinants?
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The insertional activation of J3-galactosidase enzyme, i.e. by inserting the desired gene in the coding region of enzyme, results in inactivation of (3-galactosidase gene in recombinants. The recombinant on transformed hosts are unable to produce any colour when grown on chromogenic substrate, thus acting as a selectable marker to differentiate recombinants from non-recombinants.